RNA Purifier: Preparative HPLC system for batch purification of RNA

with ternary pump, UV detection and thermal control

The therapeutic potential of mRNA is extensively discussed in times of search for vaccines in the research of COVID-19. The idea is to provide the human body with mRNA, encoding therapeutic proteins, which the immune system uses to fight the virus.¹

RNA can be produced and applied much easier compared to the full-length therapeutic proteins it is encoding for. In order to generate pure mRNA to administer, it was found that purification by chromatographic means is highly important.²

Key for RNA chromatography is the HPLC system layout. It is highly important that the chromatography is carried out at elevated temperatures in order to denature the RNA itself and hence simplify its structural diversity and yield a successful purification. Eleveated temperature in combination with a binary gradient between acetonitrile and water as done in the Reverse Phase Chromatography Method, will effectively seperate and also inactivate and unfold RNase.^3,4

The dedicated RNA Purifier is an especially designed system for RNA purification. It consists of a ternary low pressure system pump, which not only gives you a reliable formation of binary gradient between water including 1.0 M TEAA (pH 7) and acetonitrile, but also allows the direct sample application of large volume sample onto the column by the system pump via its separate sample inlet port. The flow rate range of this pump lies between 10 to 80 ml/min. Sample Injection via a sample- or Variloop is possible with the integrated injection valve. After the injection valve solvent as well as sample will be heated by an eluent heater and then applied onto the column, which additionally is heated by a heating sleeve to ensure perfect thermal purification conditions. Product identification is realized by the variable single wavelength detector at 260 nm or the wavelength of your choosing. After purification fractionation is carried out by the fractionation valve.

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Automatic sample preparation system Access Array™

CHARACTERISTICS:

Operation: Automatic

Applications: Laboratory, NGS

Sample type:for DNA

Configuration: Benchtop

Preparation format: Microarrat slide

DESCRIPTION:

Targeted DNA NGS library preparation Automate amplicon-based library preparation with the Access Array™ system for up to 48 samples per processing run. Access Array generates next-generation sequencing (NGS)-ready libraries compatible with multiple sequencing platforms including Illumina®, Ion Torrent™, and other NGS systems. Multiple chemistry options are enabled on Access Array, including Targeted DNA Seq Library Preparation reagents and Access Array reagents. Specific Enjoy high-quality results with 95% of amplicons mapped to targets. Sensitive Get the results you want from only 50 ng of input DNA. Accurate Reduce errors with a streamlined workflow and only 20 minutes of hands-on time. Compatible Work on all major next-generation sequencing instruments, like the HiSeq®, MiSeq™ and Ion PGM™.

Automated sample preparation system TrayMix™ S4

CHARACTERISTICS:

Operation: Automatic

Applications: Laboratory, for research, diagnostic, cytogenetic

Sample type: Washing, for hybridization

Configuration: Benchtop

Preparation format: for microscope slides, biochip

DESCRIPTION:

Fully Automated Hybridization Station The automated hybridization and wash station TrayMix™ S4 is a micro-mixer using a patented active mixing by Chaotic Advection technology which belongs to BioTray®. Best results are obtained thanks to an homogeneous dispersion of molecules over the whole hybridization area (21 x 60 mm). The patented Active Mixing by Chaotic Advection system achieves a significant reduction of hybridization times with the use of lower samples quantity (5 pmoles) ; a higher specificity can be reached (high reduction of background noise, increased fluorescent signal intensities). The patented Active Mixing by Chaotic Advection system eliminates artefacts such as dead zones or bubbles usually observed with other systems so that you can succeed in your hybridization and wash experiments from the start.

Nucleic acid extraction sample preparation system AU1001-96

CHARACTERISTICS:

Operation: Automatic

Applications: Laboratory, cell,whole blood, diagnostic, biological

Preparation type: Purification, cooling, heating, nucleic acid extraction, sterilization

Sample type: Cell, tissue, plasma, environmental, for biological samples, for DNA, for DNA primers, for RNA, clinical, sputum, sperm

Configuration: Benchtop

Preparation format: High-throughput, with touchscreen, high-speed

DESCRIPTION:

BioTeke’s Automated Nucleic Acid Extraction System uses cutting-edge magnetic bead separation technology to achieve fast and easy operation of DNA/RNA automation extraction procedure. All separation and purification procedures are completed in 96 well plates and there is no need for steps like regular centrifugation and filtering required by extraction and purification. The system can complete fast extraction and purification process from 1 to 32 samples, including but not limited to blood, tissue, cells, swab, virus and other clinical or forensic samples. The extracted high quality DNA/RNA is ready for use in highly sensitive downstream analysis, such as qPCR, clinical molecular diagnostic, analysis of gene expression, gene analysis, forensic and infectious disease research etc. The purified nucleic acid can be applied directly for the next enzyme cleavage, identification as well as disease diagnosis and treatment etc. As a famous domestic nucleic acid separation and purification technology company, BioTeke provides over 100 kinds of extraction kits for use with this instrument to maximize its application and minimize its costs. Features 1. Large screen English operation panel: 2. Precise control 3. Temperature control 4. Free programming 5. High purity & high yield 6. Stable results.

PCR preparation system Xdrop™

CHARACTERISTICS:

Operation: Automatic

Applications: Laboratory, for PCR, NGS

Sample type: for DNA

Preparation format: droplets

Configuration: Benchtop

Preparation format: High-throughput, with touchscreen, high-speed

DESCRIPTION:

The Xdrop instrument is a droplet generator that can produce droplets with use of specific microfluidics double emulsion and single emulsion cartridges. It is operated from the touch screen and droplet generation is fully automated. The choice of cartridge determines the size and properties of the droplet. In this way samples can be partitioned in droplets as standalone compartments for reactions. A white gasket (rubber cover) provides an air-tight seal between the instrument and cartridge during operation. Specifications Width: – 25 cm – 9,8 inches Height: – 25 cm – 9,8 inches Length: – 48 cm – 18,9 inches Weight: – 17 kg – 37,5 lbs. Voltage requirements: – 110 V-240 V Cartridges Samplix has developed advanced microfluidics easy-to-use Xdrop cartridges for droplet generation in the Xdrop instrument. Double emulsions cartridge [called dPCR cartridge] This cartridge produces double emulsion droplets, which can be used as compartments for high-throughput screening and identification of the target. Encapsulate millions of molecules or small cells (up to 6µmdiameter) with Xdrop. Run 8 samples simultaneously or individually 40 minutes of droplet generation time Produce 4-10 million droplets per lane Contamination-free workflow Single emulsions cartridge [Called dMDA cartridge] This cartridge produces single emulsion droplets, which can be used as compartments for single molecule amplification. Run 8 samples simultaneously or individually 40 seconds of droplet generation time Produce > 50,000 droplets per sample and per run Contmination-free workflow.